Thursday, January 29, 2009

Modulation of Blood Pressure by Central Melanocortinergic Pathways

Jerry R. Greenfield, M.D., Ph.D., Jeffrey W. Miller, M.D., Julia M. Keogh, B.Sc., Elana Henning, B.Soc.Sc., Julie H. Satterwhite, Ph.D., Gregory S. Cameron, Ph.D., Beatrice Astruc, M.D., John P. Mayer, Ph.D., Soren Brage, Ph.D., Teik Choon See, M.D., David J. Lomas, M.D., Stephen O'Rahilly, M.D., and I. Sadaf Farooqi, M.D., Ph.D.

ABSTRACT

Background Weight gain and weight loss are associated with changes in blood pressure through unknown mechanisms. Central melanocortinergic signaling is implicated in the control of energy balance and blood pressure in rodents, but there is no information regarding such an association with blood pressure in humans.

Methods We assessed blood pressure, heart rate, and urinary catecholamines in overweight or obese subjects with a loss-of-function mutation in MC4R, the gene encoding the melanocortin 4 receptor, and in equally overweight control subjects. We also examined the effects of an MC4R agonist administered for 7 days in 28 overweight or obese volunteers.

Results The prevalence of hypertension was markedly lower in the MC4R-deficient subjects than in the control subjects (24% vs. 53%, P=0.009). After the exclusion of subjects taking antihypertensive medications, blood-pressure levels were significantly lower in MC4R-deficient subjects than in control subjects, with mean (±SE) systolic blood pressures of 123±14 mm Hg and 131±12 mm Hg, respectively (P=0.02), and mean diastolic blood pressures of 73±10 mm Hg and 79±7 mm Hg, respectively (P=0.03). As compared with control subjects, MC4R-deficient subjects had a lower increase in heart rate on waking (P=0.007), a lower heart rate during euglycemic hyperinsulinemia (P<0.001), and lower 24-hour urinary norepinephrine excretion (P=0.04). The maximum tolerated daily dose of 1.0 mg of the MC4R agonist led to significant increases of 9.3±1.9 mm Hg in systolic blood pressure and of 6.6±1.1 mm Hg in diastolic blood pressure (P<0.001> with placebo. Differences in blood pressure were not explained by changes in insulin levels; there were no significant adverse events.

Conclusions Results of our genetic and pharmacologic studies implicate melanocortinergic signaling in the control of human blood pressure through an insulin-independent mechanism.

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